Abstract
The transport of nucleobases and nucleosides in protozoan parasites is known to be performed by Equilibrative Nucleoside Transporter (ENT) family members, including the extensively studied P1 and P2 nucleoside transporters of T. brucei bloodstream forms. Studies with P2 knockout parasites suggested the existence of as yet uncharacterised purine transport mechanisms in these cells. Here, we deleted several ENT genes, in addition to P2, including an array comprising three genes encoding for high-affinity broad-selectivity nucleobase transporters - the longest multi-gene locus deletion in T. brucei to date. It was verified that none of them appreciably contributed to the transport of hypoxanthine in bloodstream forms grown axenically in HMI-9 medium, which was mainly performed by a previously not described hypoxanthine-specific transporter (HXT1) with a K-m of 22 +/- 1.7 mu M and V-max of 0.49 +/- 0.06 pmol(10(7) cells)(-1) s(-1). The uptake of adenine was also assessed in the knockout cells and was performed by a highly specific adenine transporter (ADET1) with a K-m of 573 +/- 62 nM and V-max of 0.23 +/- 0.06 pmol(10(7) cells)(-1) s(-1). Neither HXT1 nor ADET1 displayed any affinity for other natural purines or pyrimidines and could not be completely inhibited by hypoxanthine or adenine analogues. These carriers may be the final pieces in the substantial transporter array trypanosomes can employ to fine-tune the uptake of purines from diverse environments during their life cycles and may be encoded by genes other than those of the ENT family.