Abstract
Halting the tumor initiation process by targeting the inhibition of the carcinogens metabolic activators (CYP), the induction of the carcinogen detoxification enzymes (gluthathione-S-transferases, GSTs), and the induction of antioxidant activity is an effective,c strategy. Since several dihydropyrimidine derivatives (Biginelli compounds) are therapeutically active, the Present study aimed to synthesize some dihydropyrimidines with multifunctional aromatic Substitutions and to investigate their effects as anti-initiating agents. Twelve compounds were synthesized and structually elucidated. The results revealed that compound 10 was a non-cytotoxic inhibitor of cytochrome P 450 1A (Cyp1A) activity, inducer of GST activity, scavenger of OH and inhibitor of DNA fragmentation. Compounds I and 9 were radical scavengers of OH and inhibitors of DNA fragmentation. On the other hand, all compounds were not toxic against different tumor cells, except compounds 2, 4, and 5 possessed non specific cytotoxicity against both liver and colon carcinoma cells, while 7 possessed specific cytotoxicity only against colon carcinoma cells. Compound 1 was a non-cytotoxic inducer of GST GST activity, scavenger of OH and ROO, and inhibitor of DNA fragmentation. The present study proved that compounds 10 and I were active and safe turner anti-initiating and multi-potent blocking agent.