Abstract
We report a rapid and facile method for detection of hydrogen peroxide (H2O2) in biological fluids using sub-stoichiometric two-dimensional (2D) molybdenum trioxide (α-MoO3-x) nanoflakes. The two-dimensional nanoflakes, initially blue in color, is oxidized after interaction with hydrogen peroxide thereby changing its oxidation state to form α-MoO3. The change in oxidation state of nanoflakes transforms from blue to a visually distinct hazy blue color with change in absorption spectrum. The phenomenal property is explored here in sensing up to 34 nM as limit of detection. The efficacy of the detection system was analyzed by “zone of inhibition” based agar diffusion assay with different concentrations of H2O2. The current approach is highly accurate, effective and reproducible for quantification of physiological concentration of H2O2 in biological fluid such as human urine.
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•We have developed a bare eye sensor for Hydrogen peroxide.•α-MoO3-x is used as Rapid detection probe for Hydrogen peroxide.•The method has also been tested for the first time on biological fluids to affirm the sensitivity in real samples.•It has also been tested on agar well diffusion assay detection based on its “zone of inhibition”.