Abstract
Present study reports metabolites profiling, antioxidant, and antidiabetic effects ofCuscuta reflexagrown onCasearia tomentosa. The UHPLC-QTOF-MS/MS method was used to characterize phytochemicals in most potent extract. Thirteen bioactive compounds/metabolites including (2S)-3-(beta-D-galactopyranosyloxy)-2-(hexanoyloxy)propyl nonanoate, 4-O-beta-D-glucosyl-4-coumaric acid, isorhamnetin-3-O-glucoside, 7-(alpha-D-glucopyranosyloxy)-2,3,4,5,6-pentahydroxyheptanoic acid, 13S-hydroxyoctadecadienoic acid, astragaloside derivative, salicylic acid, oleanolic acid, citric acid, pinellic acid, quinic acid, caffeic acid derivative and caffeic acid-3-glucoside were detected in 80% hydroethanolic (HE) extract. Among different extracts (aqueous, 20-80% HE and pure ethanolic), 80% HE extract showed maximum TPC (186.19 +/- 2.15 mg GAE/g DE) and TFC (106.50 +/- 1.68 mg RE/g DE). A strong correlation was observed between TPC, TFC, DPPH radical scavenging (IC50 = 60.64 +/- 1.74 mu g/mL) and TAP activity (235.96 +/- 1.33 mg AAE/g DE) of 80% HE extract among others. The same extract also depicted highest alpha-amylase and alpha-glucosidase enzyme inhibition with IC(50)of 71.84 +/- 1.06 and 57.25 +/- 1.40 mu g/mL, respectively. Molecular docking was performed to explore possible role of identified phytochemicals. Binding affinity data as well as interaction patterns have revealed possible interaction of the identified bioactives against alpha-glucosidase and alpha-amylase. Moreover, intra-molecular charge transfer, energies of the FMOs, HOMO-LUMO-E-gaps, MEP and IP were also explained. The present findings suggested that,C. reflexais a promising natural antioxidant and antidiabetic agent, thus supporting the use of this plant for designing related functional foods and nutra-pharmaceuticals.