Abstract
Metformin hydrochloride and glimepiride are commonly combined in one tablet for the Management of type 2 diabetic patients. This study offers a tandem mass spectrometric method for the concurrent quantification of the studied drugs in plasma. They were extracted on using n-heptane: propanol (3: 2,v/v) and chromatographed on Waters RP-C18 column. The mobile phase was water containing 0.1% formic acid: methanol (3: 7, v/v) as a mobile phase. The linearity ranges were 50 - 2000 ng/mL for metformin hydrochloride and 0.1 - 1000 ng/mL for glimepiride. Validation process was fully carried out for the proposed method. This work can be applied for the estimation of the studied drugs for bioavailability and pharmacokinetic studies.