Abstract
•Nanoporous GR-Fe3O4/MWCNTs-Fe3O4/NP-PtTi composite based high sensitive and selective Penicillin sensor.•The linear detection range of the fabricated sensor was from 0.05 to 100 ng mL−1.•A low detection limit of 25.3 pg mL−1 was observed from the fabricated sensor.•The designed aptasensor possess potential application on penicillin detection in real samples.
Herein, we report the fabrication of aptamer probe for ultrasensitive detection of penicillin based on unique nanocomposite material consist of graphene and ironoxide (magnetite) (GR-Fe3O4) and multi-walled carbon nanotubes and ironoxide (MWCNTs-Fe3O4) with nanoporous platinumand titanium(NP-PtTi) alloy. The prepared new nanocomposite material provide a signal amplification and immobilization strategy and hence significantly contribute in the fabrication of ultrahigh sensitive label-free penicillin aptasensor. The detailed studies confirmed that the prepared newly composite i.e. nanoporous PtTi/GR-Fe3O4/MWCNT-Fe3O4 exhibited improved synergistic effect compared with any other single material. The developed biosensor demonstrates ideal stability and reproducibility with high selectivity. The observed linear range of the as-prepared aptasensor was from 0.05 to 100 ng mL−1 with an ultra-low detection limit of 25.3 pg mL−1. Based on the good sensing performance, the constructed electrochemical aptasensor showed potential practical application in the quantitative analysis of penicillin.