Abstract
Cancer growth is a developing significant public health threat, and notwithstanding the advances in biomedical research and innovation, a pressing need is felt for the progress of new anticancer drugs. Sulfated polysaccharides, such as ulvan from green macroalgae, exhibit a diverse range of biological applications. In this study, a novel d-mannose-mediated targeted drug delivery system (GO–CH–Ma) for targeting glioblastoma cancer was developed by loading Ulvan lactua as the anticancer model drug onto functionalized graphene oxide. Ultraviolet spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and Zeta potential were used to describe the main physicochemical properties of the chitosan-functionalized graphene oxide (GO-CH). Similarly, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) enabled the study of surface morphology. The entrapment of ulvan on GO–CH–Ma has been observed to be 88%. The biocompatibility of the nanocarrier and drug-loaded nanocarrier was studied via hemolysis and anti-inflammatory assay. The in vitro drug release profile of ulvan revealed a pH-dependent-controlled release system observed by UV–Visible analysis. Moreover, a human glioblastoma cell line (U87) was used to examine the preliminary in vitro cytotoxicity. Finally, a mannose-decorated GO-CH carrier loaded with ulvan demonstrated a promising targeted drug delivery system to treat in vitro glioblastoma.
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•Ulvan is used as an anticancer model drug and an excellent candidate for biomedical fields.•Surface modifications of graphene oxide with polysaccharides (GO–CH–M) were successfully constructed.•Ulvan loaded GO-CH-M nanoparticles show enhanced biocompatibility and pH-sensitive drug delivery efficiency.•In-vitro cell cytotoxicity studies were carried out on glioblastoma cell lines.