Abstract
Nanostructured materials have been extensively exploited in the food sector for nutrient delivery, sensing, packaging, and food additives. It has exhibited size, shape, chemical nature, and surface area-dependent physicochemical and biological properties. Currently, nanoscale ingredients are identified in approved food additives. Still, food-grade nanostructured material's impact on biological systems is unexplored well. Thus, in this study, we have identified and analyzed the physicochemical properties of nanoscale ingredients in commercial food product isolate E341 and E171 combinations. Also, we have evaluated the E341/E171 combination cytotoxic potential on human mesenchymal stem cells. The food isolated E341/E171 exhibits spherical nature with a 60-200 nm diameter. The E341 and E171 combination exposure increased the cell viability loss to -36% at a high dose in human mesenchymal stem cells (hMSCs). The E341/E171 treatment-induced cellular and nuclear damage at a moderate dose. The mitochondria! membrane potential loss and ROS generation were observed in E341/E171 treated cells. The E341/E171 alters the gene expression pattern in hMSCs dose-dependently. The GSR and POR gene expression is upregulated significantly in E341/E171 treatment. Our study results revealed that E341/E171 induced toxicity in hMSCs at high concentrations. Thus, E341/E171 potential impact on the biological system should be reexamined for food industrial implications.