Abstract
A simple isocratic reversed phase high performance liquid chromatographic (HPLC) method using C18 column with ultraviolet detection at 225 ism has been described for quantitative determination of gliquidone in formulation and human serum. The system is operated at room temperature using a mobile phase consisting of methanol: water (90:10) adjusted to pH 3.50 with phosphoric acid with a flow rate of 1 mLmin(-1). Methylparaben is successfully used as an internal standard. The assay is reproducible, linear (concentration range of 0.60-25 mu gmL(-1)) with correlation coefficient of 0.9999 and accurate (99.23 to 102.78%). The intra and inter run precision results are 1.14 and 1.32%. The LOD and LOQ values are 0.03 and 0.6 mu gmL(-1), respectively. There are no interfering peaks due to the excipients present in the pharmaceutical tablet. Thus, the proposed method is simple and suitable for the analysis of active ingredient in tablet dosage form and human serum.