Abstract
A rapid high performance liquid chromatographic method was developed and validated for the determination and stability evaluation of linezolid in pharmaceutical dosage forms. Separation of linezolid was successfully achieved on a C-18 column utilizing water: methanol (50:50 v/v) at a flow rate of 1 mL/min and eluate was monitored at 254 nm, with a retention time of 5.117 minute. The method was validated and the response was found to be linear in the drug concentration range of 0.001 mg/mL to 3.4 mg/mL. The mean values ± RSD of the slope and the correlation coefficient were found to be 51169 ± 0.290 and 0.9999, respectively. The RSD values for intra- and inter-day precision were found to be 0.782 (<1.00%) and 0.610 (<0.90%), respectively. The forced degradation kinetic study of linezolid by using HCl (0.5 N), NaOH (0.02 M), H2O2 (6%v/v), thermal (80 ± 2°C), and UV radiation (365 nm) were observed to be very specific.