Abstract
The aim of this study is to develop a simple and applicable HPLC method for the detection of cefpodoxime acid (CFA) in rabbit plasma after oral administration of cefpodoxime proxetil (CFP) loaded chitosan-alginate (CH-ALG) beads formulation. CFP is a prodrug that is deesterified in vivo to its active metabolite CFA to exhibit antibiotic activity. Chromatographic separation of CFA and internal standard (IS) was achieved by a RP18(C18), Phenomenax (R) 100, (250 x 4.6 mm) with the mobile phase consisting of (0.02 mol/l (20 mM) ammonium acetate solution and acetonitrile (92:8, v/v, pH =4.6) at a flow rate of 1.0 ml/min. The method was validated according to the requirements of US-FDA guidelines for bio-analytical method validation. The linear regression analysis for the calibration plots showed good linear relationship (R-2 = 0.9905) in the working concentration range of 0.5-50 mu g/ml. The limits of detection and quantification (S/N = 3) were 0.069 and 0.136 mu g/ml. Plasma CFA levels were successfully determined in rabbit with satisfactory precision and accuracy. The analyte was found to be stable after a number of stability studies. The proposed HPLC method was successfully applied to pharmacokinetic study in rabbits for CFP loaded CH-ALG beads and marketed immediate release (IR) tablets. All pharmacokinetic parameters were assessed. (C) 2017 Elsevier B.V. All rights reserved.