Abstract
Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing β serine recombinase acting on
six
recognition sequences (β-rec/
six
) in a fungal host, the human pathogen
Aspergillus fumigatus
, and its use in establishing a self-excising resistance marker cassette for serial gene replacement.