Abstract
Screening for the important variables is part of the optimization study of enzyme production. It is important indetermining the influential variables that give a significant effect on the enzyme production. Thus, the objective of this study was to determine variables that are effecting the recombinant phytase production from Escherichia coli DH5 +/-. Through the application of the Fractional Factorial Design, the variables of seed age, inoculum level, time of induction, L-arabinose concentration and post-induction time were highly significant for the recombinant phytase production as their p-values were less than 0.05.Thus, minimal changes of this factor mat effect recombinant phytase production from E. coli DH5 +/-.