Abstract
Glucosinolate (GSL) contents in five varieties and eleven lines of rocket salad (Eruca sativa L.) were quantified using HPLC-UV at 227 nm. Eleven GSLs including five aliphatic (glucoraphanin, glucothiobeinin, glucobrassicanapin, glucoerucin, and dimeric 4-mercaptobutyl GSL), two indolyl (glucobrassicin and 4-methoxyglucobrassicin), one aromatic (gluconasturtiin) and three unknown GSLs were identified based on our data base. Aliphatic GSLs were noted as the predominant group with an average 89% of the total content. The highest total GSL amounts were documented in 'Herb-Fragrant Vegetable' (31.12 mu ol center dot g(-1) dry weight (DW)), whereas the lowest was in line 28612 (9.91). Relatively 73% of lines among the lines documented the content more than 8 mu mol center dot g(-1) DW of dimeric 4-mercaptobutyl GSL, particularly lines 28615 and 28619 noted more than > 17 mu mol center dot g(-1) DW. The sum of three major GSLs (glucoraphanin, glucoerucin, and dimeric 4-mercaptobutyl GSL) attributed > 8 mu mol center dot g(-1) DW in all rocket salads, especially 'Herb-Fragrant Vegetable' and line 28613 (87 and 93% respectively) in the total GSL accumulation, likewise line 28612 and 28620 attributed > 90%. Indolyl GSLs were ranged 0.27-1.09 mu mol center dot g(-1) DW, accounted less than 6% of the total GSLs in varieties and lines. These results provide valuable information regarding the potential beneficial GSL contents individually in different varieties and lines of rocket salads.