Abstract
The acute toxicity from volatile toxin from the Limonia acidissima (La-VO) was evaluated on the mosquito, Aedes aegypti. Screening of La-VO showed five major bioactive compounds with a major peak estragole. The mortality rate was significantly greater at 150 ppm La-VO. The lethal concentration (LC50) was observed at 65.24 ppm. Sub-lethal dosage of La-VO resulted in significant changes in developmental times. Enzymatic profile of larvae post treatment showed upregulation of CYP450 and GST levels. A maximum repellent activity was observed at 75 ppm La-VO at 98% up to 210 minutes. The La-VO provided evidence of insecticidal and toxicity to the mosquito.