Abstract
Presence of Wheat dwarf India virus (WDIV) and Ageratum yellow leaf curl betasatellite (AYLCB) was confirmed using polymerase chain reaction and sequencing in wheat samples originating from Pakistan for the first time. However, no alphasatellites were detected from the analyzed samples. Both, WDIV and AYLCB were highly similar to their counterparts reported from wheat in India. The findings of this study suggest a strong association between WDIV and AYLCB. This is also the first report of a monocot infecting mastrevirus and the presence of a satellite in a monocot plant in Pakistan.