Abstract
A chromone glucoside 2-methyl-5,7-dihydroxychromone 5-
O
-β-D-glucopyranoside (schumanniofioside A, compound 1) was isolated from the methanol extract of
Acalypha fruticosa
. The structure of compound 1 was fully assigned based on nuclear magnetic resonance (NMR) (
1
H,
13
C and 2D) spectra and electrospray ionization mass spectrum (ESI-MS) in addition to X-ray Crystallography. The molecules were packed in the crystal structure by eight intermolecular O—H⋯O and C—H⋯O interactions. The structure of compound 1 belongs to monoclinic,
P
2
1
,
a
= 9.1989 (4) Å,
b
= 4.6651 (2) Å,
c
= 20.4042 (7) Å, β = 97.862 (3)°,
V
= 867.31 (6) Å
3
,
Z
= 2,
wR
ref
(F
2
)
= 0.101,
T
= 100 K. Thus, the bond angles, bond lengths and absolute structure of compound 1 were confirmed by its X-ray structure. A validated HPTLC method was developed for the quantitative analysis of compound 1 in chloroform and methanol extracts of
A. fruticosa
. It was found to furnish a compact and sharp band of compound 1 at R
f
= 0.13 ± 0.005 using chloroform, methanol and glacial acetic acid [17:3:0.5 (v/v/v)] as mobile phase. The LOD and LOQ for compound 1 were found to be 17.86 and 54.13 ng/band, respectively. Compound 1 was found in both chloroform and methanol extracts of the plant (0.03% w/w and 0.31% w/w, respectively). The proposed HPTLC method can be used for the further analysis of schumanniofioside A in different plant extracts, herbal formulations and biological samples as well as in process quality control.