Abstract
Xenobiotic thiencarbazone-methyl is a herbicide applied on various grasses and weeds. This triazolinone pesticide may be present in soil and water after application posing deleterious effects on environment and human health. The degradation of thiencarbazone-methyl was scrutinized by the pure bacterial strains includingStreptococcus pneumoniae(SP),Escherichia coli(EC) andStreptococcus pyogenes(SPy). Individual bacterial suspension was prepared with herbicide solution and evaluated for a duration of 28 days. Time-dependent sampling was performed to investigate thiencarbazone-methyl degradation after specific days interval. Biotransformed samples were extracted and analyzed by UV-Vis spectrophotometer and GC-MS. Biodegradation percentage achieved by SP, Spy and EC, after 28 days, was 31, 40 and 65, respectively.E. coli(R-2= 0.91) displayed greatest potential among all the strains to degrade the herbicide. Significant residues formed included methyl 4-isocyanatosulfonyl-5-methylthiophene-3-carboxylate, 4-methanesulfonyl-benzenesulfonic acid amide and methyl 3-sulfamoylthiophene-2carboxylate. Current research elucidated the effectiveness of using bacterial strains for the bioremediation of pesticides in environment.