Abstract
A new matrix for enzymes covalent immobilization has been prepared by the activation of alginate's CH groups using p-Benzoquinone. The immobilization of p-Galactosidase onto the surface of p-Benzoquinone activated alginate beads has been presented as strategy to overcome the diffusion limitation problem of the substrate. Factors affecting the activation process and its impact on the activity of immobilized enzyme have been studied. Changes in the temperature and pH profile for the immobilized form in comparison with the free form have been observed. Moreover, the effect of the immobilization on the kinetic parameters of the immobilized enzyme has been monitored. No signals of substrate diffusion limitation have been detected.