Abstract
Significant amount of studies demonstrated that the abnormal expression of microRNAs were involved in the carcinogenesis and progression of various kinds of human cancers, also including osteosarcoma. Therefore, understanding the physiological and molecular mechanisms of miRNAs in osteosarcoma may provide novel therapeutic strategies for treatments of patients with osteosarcoma. In this study, we investigated the expression, biological roles of microRNA-486 (miR-486) on osteosarcoma and its underlying mechanism. The results showed that miR-486 was significantly down-regulated in osteosarcoma tissues and osteosarcoma cell lines. In addition, overexpression of miR-486 inhibited cell proliferation, migration and invasion of osteosarcoma. Moreover, SIRT1 was identified as a direct target gene of miR-486 in osteosarcoma. SIRT1 knockdown could mimics the functions of miR-486 over-expression in osteosarcoma cells. In conclusion, the data of the present study provides compelling evidences that miR-486 acted as a tumor suppressor in osteosarcoma through targeting SIRT1 and could serve as a novel therapeutic target.