Abstract
Diabetes mellitus is a metabolic disorder and is a global challenge to the current medicinal chemists and pharmacologists. This research has been designed to isolate and evaluate antidiabetic bioactives from
. The crude extracts, semi-purified and pure bioactives have been used in all in vitro assays. The in vitro α-glucosidase, α-amylase and DPPH free radical activities have been performed on all plant samples. The initial activities showed that ethyl acetate (
) was the potent fraction in all the assays. This fraction was initially semi-purified to obtain
-
. Among the semi-purified fractions,
was dominant, exhibiting potent IC
values in all the in vitro assays. Based on the potency and availability of materials,
was subjected to further purification to obtain compounds
(2,4-dichloro-6-hydroxy-3,5-dimethoxytoluene) and
(2-methyl-6-(4-methylphenyl)-2-hepten-4-one). The two isolated compounds were characterized by mass and NMR analyses. The compounds
and
showed excellent inhibitions against α-glucosidase (21.45 for
and 15.03 for
μg/mL), α-amylase (17.65 and 16.56 μg/mL) and DPPH free radicals (7.62 and 14.30 μg/mL). Our study provides baseline research for the antidiabetic bioactives exploration from
. The bioactive compounds can be evaluated in animals-based antidiabetic activity in future.