Abstract
[EN] A sensitive, simple and label-free immunosensor for atrazine detection was developed. The immunosensor was based on antibody physisorption as immobilization technique. This technique was proposed as a cost-effective gold immuno-functionalization strategy alternative to SAM-based strategy for pesticide detection. Each step of immunosensor construction was characterized by electrochemical impedance spectroscopy, and the antibody-atrazine interaction was measured in a dynamic range from 10 to 150 ng/mL. This method was highly selective for atrazine and propazine, showing weak or no cross-reactivity to other similar compounds like terbutylazine. A limit detection of 10 ng/mL of atrazine was detected in PBS buffer with a good reproductibility. Lower limit detection in tap water was obtained.
This work was financially supported by the PCI cooperation project between Spain and Tunisia. The authors thank Dr. F. Del Campo and Dr. E. Baldrich from CNM-Barcelona (Spain) for the interdigitated microelectrode fabrication. Pr. A. Abdelghani has to thanks the NATO (North Atlantic Treaty Organization) for the material donation.
Marrakchi, M.; Campos Sánchez, I.; Helali, S.; Mejri, N.; Soto Camino, J.; González Martínez, MÁ.; Hamdi, M... (2011). A Label-Free Interdigitated Microelectrodes Immunosensor for Pesticide Detection. Sensor letters. 9:2203-2206. doi:10.1166/sl.2011.1800
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