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Diffraction tomography for biological cells imaging using digital holographic microscopy
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Diffraction tomography for biological cells imaging using digital holographic microscopy

Isabelle Bergoënd, Cristian Arfire, Nicolas Pavillon and Christian Depeursinge
LASER APPLICATIONS IN LIFE SCIENCES, Vol.7376(1), pp.737613-737618
SPIE
01/01/2010

Abstract

Optics Physics
Many biological objects are mainly transparent and weakly scattering, thus a promising (and already widely used) way of imaging them consists in considering optical refractive index variations. The method proposed here permits 3D imaging of the refractive index distribution with a tomographic approach. Usually, the classical Radon transform does not sufficiently take into account the physical interaction between light and biological cells, therefore diffraction has to be considered.

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